Figure 2.
Visualization of added maize chromatin and chromosomes in oat background. Two examples are shown for GISH by hybridizing fluorescein-labeled maize DNA without unlabeled competitor DNA to root tip meristem cells in interphase and in metaphase. Oat chromosomes are counterstained with propidium iodide (red) and maize chromosomes are yellow-green because of hybridized fluorescein-labeled probe. A, OMAm5.9, an F1 plant with monosomic addition of maize chromosome 5, illustrating that maize chromatin can be scored in F1 interphases. B, OMAd1.7, disomic addition of maize chromosome 1 to hexaploid Starter oat.