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Identification of added maize chromosomes by SSRs. PCR products of genomic DNA of a selection of 10 maize-positive oat × maize F1 plants (A–J), oat, and maize are shown after electrophoresis in 4.5% (w/v) agarose gel. Bands represent DNA fragments that were amplified with SSR markers specific for one of the 10 maize chromosomes. Maize specificity is shown by presence in maize DNA and absence in oat DNA or by length polymorphisms between maize and oat.
