Table 1.
Phenotypic and genotypic characterization of extended-spectrum cephalosporin resistant Escherichia coli isolates obtained from companion animals clinical specimens.
| Phenotype | β-lactamases enzyme | Number of isolates | Phylogroup | ST | Year of isolation | Source | Body site |
|---|---|---|---|---|---|---|---|
| ESBL (n = 53) | CTX-M-15 | 7 | A, B1 (n = 4) B2 (n = 2) U (n = 1) |
86 (1) 23 (1) 623 (1) 3880 (1) 4184 (1) |
2010 (n = 1), 2013 (n = 1), 2014 (n = 3) |
Dog (n = 5), Horse (n = 2) |
GI tract (n = 3), SI/O (n = 1), Urine (n = 1), Wound (n = 2) |
| CTX-M-15, OXA | 10 | A, B1 (n = 2) B2 (n = 3) C (n = 4) D (n = 1) |
131 (3) 167 (1) 1284 (1) 2348 (1) |
2010 (n = 4), 2011 (n = 3), 2012 (n = 1) |
Dog (n = 9), Horse (n = 1) |
GI tract (n = 4), Hepato-biliary (n = 1), Urine (n = 1), SI/O (n = 3), Wound (n = 1) |
|
| CTX-M-15, TEM, OXA | 2 | D (n = 1) F (n = 1) |
648 (1) | 2011 | Dog | BF/BP (n = 2), SI/O (n = 3) |
|
| CTX-M-15, TEM | 3 | A | 10 (1) 167 (1) |
2011 | Dog | Wound (n = 3) | |
| CTX-M-14 | 6 | A (n = 3) B2 (n = 2) C (n = 1) |
10 (2) 95 (1) 209 (1) |
2012 (n = 2) 2014 (n = 1), 2015 (n = 3) |
Dog (n = 2), Cat (n = 1), Horse (n = 1) |
GI tract (n = 1), SI/O (n = 1), Urine (n = 4) |
|
| CTX-M-14, TEM | 8 | A (n = 7), D (n = 1) |
10 (1) 209 (1) 617 (2) |
2010 (n = 5), 2015 (n = 2), 2016 (n = 1) |
Dog (n = 8) | BF/BP (n = 2), Hepato-biliary (n = 2) GI tract (n = 1), Urine (n = 3) |
|
| CTX-M-55 | 2 | B2 (n = 2) | 1340 (2) | 2015 (n = 2) | Dog (n = 2) | GI tract (n = 2) | |
| CTX-M-55, TEM | 3 | A (n = 1) B2 (n = 2) |
167 (1) 1340 (2) |
2015 (n = 2), 2016 (n = 1) |
Dog (n = 3) | Urine (n = 2), GI tract (n = 1), |
|
| CTX-M-55, OXA | 1 | B2 | 131 | 2015 | Dog | Urine | |
| CTX-M-55, TEM, SHV-12 | 1 | B1 | 5036 | 2013 | Horse | Wound | |
| CTX-M-1 | 3 | B1 (n = 2) D |
47923509 | 2013 (n = 2) 2014 (n = 1) |
Dog | GI tract | |
| CTX-M-1, TEM | 1 | B1 | 641 | 2013 | Horse | Wound | |
| CTX-M-27 | 1 | B2 | 131 | 2010 | Dog | Hepato-biliary | |
| CTX-M-28, TEM | 3 | B1 (n = 3) | 3743448 (2) | 2015 | Dog (n = 2), Horse (n = 1) |
SI/O (n = 2), Wound (n = 1) |
|
| CTX-M-24, TEM | 1 | A | – | 2014 | Dog | Wound (n = 1) | |
| CTX-M-24, OXA | 1 | C | 3163 | 2012 | Dog | Wound (n = 1) | |
| AmpC (n = 67) | CMY-2 | 23 | B1 (n = 4) B2 (n = 9) C/F (n = 3) D (n = 7) |
– | 2010 (n = 2), 2011 (n = 1), 2012 (n = 6) 2013 (n = 4), 2014 (n = 5), 2015 (n = 4), 2016 (n = 1) |
Dog (n = 23) | Wound (n = 7), Urine (n = 6), GI tract (n = 5), SI/O (n = 4), Hepato-biliary (n = 1) |
| CMY-2, TEM | 30 | A/B1 (n = 12) B2 (n = 12) C (n = 3) F (n = 3) |
2348 (1) | 2010 (n = 1), 2011 (n = 2), 2012 (n = 12) 2013 (n = 4), 2014 (n = 4), 2015 (n = 6) |
Dog (n = 28), Cat (n = 1), Horse (n = 1) |
Urine (n = 15), GI tract (n = 5), Wound (n = 4), BF/BP (n = 3), Hepato-biliary (n = 2), SI/O (n = 1) |
|
| CMY-2, TEM, OXA | 3 | B2 (n = 1) C (n = 1) D (n = 1) |
410 (1) | 2012 (n = 1), 2013 (n = 2) |
Dog | Urine (n = 1), SI/O (n = 1), GI tract (n = 1) |
|
| CMY-2, OXA | 2 | A (n = 1) C (n = 1) |
– | 2010 (n = 1), 2012 (n = 1) |
Dog (n = 2) | Wound (n = 2) | |
| CMY-2, TEM, DHA | 1 | B2 | – | 2015 | Dog | SI/O | |
| DHA | 1 | B1 | – | 2015 | Dog | Wound | |
| DHA, TEM | 3 | A/B1 (n = 2) F (n = 1) |
– | 2013 (n = 1), 2015 (n = 1), 2016 (n = 1) |
Dog | Hepato-biliary (n = 1), Urine (n = 1), Wound (n = 1) |
|
| DHA, TEM, OXA | 1 | B1 | – | 2015 | Dog | Wound | |
| EBC | 1 | B2 | – | 2014 | Horse | Wound | |
| EBC, TEM | 2 | B2 | – | 2014 | Horse | Wound (n = 2) | |
| ESBL/AmpC(n = 11) | CTX-M-15, OXA, CMY-2 | 4 | B2 (n = 1) C (n = 3) |
1485 (1) | 2012 (n = 3), 2013 (n = 1) |
Dog | BF/BP (n = 1), SI/O (n = 1), Urine (n = 1), Wound (n = 1) |
| CTX-M-15, TEM, OXA, CMY-2 | 3 | B2 | 410 (1) 3163 (1) |
2011 (n = 1), 2012 (n = 2) |
Dog | GI tract | |
| CTX-M-15, TEM, OXA, DHA | 1 | D | – | 2013 | Dog | SI/O | |
| CTX-M-24, CMY-2, TEM, OXA | 1 | U | – | 2013 | Dog | Hepato-biliary | |
| CTX-M-24, CMY-2, TEM, SHV-12 | 1 | C | 410 | 2013 | Horse | BF/BP | |
| CTX-M-55, TEM, CMY- 2 | 1 | A | 167 | 2013 | Dog | GI tract (n = 1) | |
| Inconclusive phenotype (n = 33) | TEM, OXA | 1 | B1 | – | 2014 | Dog | Bile |
| TEM | 13 | A/B1 (n = 10) B2 (n = 2) U (n = 1) |
– | 2010 (n = 2), 2011 (n = 1), 2012 (n = 1), 2013 (n = 1), 2014 (n = 3), 2015 (n = 2), 2016 (n = 3) |
Dog (n = 8), Cat (n = 2), Horse (n = 3) |
BF/BP (n = 1), GI tract (n = 2), SI/O (n = 2), Urine (n = 5), Wound (n = 3) |
|
| OXA | 4 | B1 (n = 1), F (n = 2), U (n = 1) |
– | 2012 (n = 3), 2014 (n = 1) |
Dog (n = 3), Horse (n = 1) |
Wound (n = 2), Urine (n = 1), SI/O (n = 1) |
|
| – | 15 | B1 (n = 2) B2 (n = 12) C (n = 1) |
– | 2010 (n = 1), 2011 (n = 1), 2012 (n = 1), 2013 (n = 2), 2014 (n = 3), 2015 (n = 5), 2016 (n = 2) |
Dog (n = 11), Cat (n = 4) |
Urine (n = 7), Wound (n = 3), SI/O (n = 3) Hepato-biliary (n = 2) |
|
ST, sequence type by multi-locus sequence typing; BF/BP, body fluids/biopsies; SI/O, skin infection/otitis.