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. 2019 Jun 5;10:1233. doi: 10.3389/fmicb.2019.01233

FIGURE 6.

FIGURE 6

Promoter test experiments in WT or suppressor mutant background. Promoter activities of sll1526 and sll0470 were measured in Synechocystis WT or Δsll0729::supp_15 luxAB reporter strains. (A) Promoter regions of sll1526 and sll0470 possess upstream of the transcriptional start site (TSS, +1) a GGCC motif. The information about the TSS was taken from Kopf et al. (2014). CDS, coding sequence. (B) Luciferase-mediated bioluminescence of Psll0470 or Psll1526 luxAB fusions in the WT or in the Δsll0729::supp_15 after incubation with decanal. The GGCC motif was changed to GCGC in the promoters suffixed by “m” (Psll0470m and Psll1526m). The WT without luxAB genes was used as negative control in the presence (+d) and absence (−d) of decanal. All measurements were performed in biological quadruplicates; the luminescence is given in arbitrary units (a.u.). Statistical analysis was performed using the two-tailed unpaired Student’s t-test (∗∗∗p < 0.001).