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Edu assays and protein expression of Cdc6 in BCSLCs. (A) Edu assays were conducted to evaluate DNA synthesis (proliferation ability). (B) Proportion of Edu+ cells (%). (C) Cdc6 protein levels were analyzed by Western blotting. Cdc6-S: non-chromatin binding Cdc6; Cdc6-C: chromatin-binding Cdc6. (D) Relative OD of Western blot bands is presented. (*P<0.05).
Abbreviations: BCSLCs, bladder cancer stem-like cells; Cdc6-S, non-chromatin binding Cdc6; Cdc6-C, chromatin-binding Cdc6.
