Effect of IgG anti-prothrombin antibodies on the binding of prothrombin to phospholipids. PC:PS (75:25 wt/wt) liposomes were immobilized onto an L1 chip, and proTWT was injected at a concentration of 0.5 μM (or 0.036 mg/mL) in the absence (solid green line) or presence (0.10-0.15 mg/mL, solid red or blue line) of IgGs. Panels A-C display the effect of IgGs isolated from P4, P24, and P27 in group A (solid red line). Panels D-F display the effect of IgGs isolated from P1, P10, and P25 in group B (solid blue line).