Figure 2.
Inhibition of endogenous ELF-1 decreased NPC cell growth. (A and D) Endogenous of ELF-1 protein expression was determined using an anti-ELF-1 antibody Western blotting. (B and E) Cell growth of siELF-1 NPC transfectants and the negative control were assessed using by MTT assay. (C and F) The BrdU incorporation assay was performed to determine the proliferation of siELF-1 transfectants and the negative control. The results are expressed as the mean±SD of triplicate wells in three independent experiments. ***p<0.001.
Abbreviations: BrdU, bromodeoxyuridine; MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NPC, nasopharyngeal carcinoma; SD, standard deviation.