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. 2019 Apr 30;7(5):119. doi: 10.3390/microorganisms7050119

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of BART miRNA deletion on progeny virus production and NF-κB signaling activity. (a) Loss of BART miRNA expression from HEK293 cells harboring BART(−)LMP1_CAO was verified by a PCR-based assay. The amounts of mature BART1-3p miRNA, whose gene is located outside of the BART deletion, are presented as 100. (b) Progeny viruses of BART(+)LMP1_CAO or BART(−)LMP1_CAO were used to infect EBV-negative Akata cells. The percentage of GFP-positive cells was measured by fluorescence activated cell sorting analysis. (c) LMP1 expression levels in stably transfected HEK293 cells harboring EBV-BAC clones as indicated (lanes 2 through 5). Lane 0; LCL established by B95-8 strain EBV. Lane 1; control HEK293 cell. (d) The NF-κB reporter plasmid was used to examine NF-κB signaling activities in HEK293 cells harboring various EBV-BAC clones. Lane numbers correspond to those in (c).