Figure 1.

A, Water loss assay. Detached leaves of wild type (WT) and mutant (M) were weighed every 10 min until 60 min. Mean and sd of three independent experiments are shown. B, ABA levels in WT and M at 0 h and after 3 h of drought treatment. Mean and sd of three independent experiments are shown. C, Tagging of OsABA1 by Tos17. Cosegregation analysis of XbaI-digested genomic DNA (0.5 μg) was carried out by Southern analyses, and probed with a ³²P-labeled Tos17 (left) or the cloned OsABA1 gene (right). Numbers on the top of each lane represent individual seedling, and the genotype of each seedling is also given on the top of each lane. Lane 1 carries the genomic DNA of Nipponbare and serves as a control for other seedlings. The size of marker (λDNA digested with HindIII) fragments is indicated on the left hand in kilobase pairs.