Figure 2.

Structural features of the OsABA1 and position of Tos17 insertion. A, Diagrammatic representation of the OsABA1 ORF showing the initiation codon (ATG) and the stop codon (TGA), and the positions of Tos17 insertion. Nucleotides of OsABA1 flanking the Tos17 insertion or an intron are also given. Hatched lines indicate the putative chloroplast transit peptide. B, Four regions of the tobacco ZEP (ABA2) protein are compared with the OsABA1 protein. The identical amino acid is marked by asterisk. C, Northern analysis of OsABA1 in wild type and mutant (Osaba1). Total RNA (20 μg) was subjected to northern analysis and probed with the ³²P-labeled OsABA1. Equal loading was confirmed by staining with ethidium bromide.