Figure 5.

A, Diagrammatic representation of the OsTATC ORF showing the translation start point (ATG) and the stop codon (TGA), and the position of Tos17 insertion. Nucleotide of OsTATC flanking the Tos17 insertion is given. The putative chloroplast transit peptide (hatched lines), mitochondrial targeting peptide (horizontal lines), and six transmembrane helices (clear areas) are also shown. B, Hydrophilicity analysis of OsTATC protein. Hydrophilicity was analyzed using the Tmpred program. C, Northern analysis of OsTATC in wild type and mutant (Ostatc). Total RNA (20 μg), isolated from leaf and shoot of two individual seedlings, was subjected to northern analysis, and probed with the ³²P-labeled OsTATC. Equal loading was confirmed by staining with methylene blue.