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Flow cytometry of L. lactis cells displaying or secreting FLAG-tagged REX variants, detected with an anti-FLAG antibody. Control: L. lactis containing an empty plasmid of pNZ8148. (A) Mean fluorescence intensity (MFI) or (B) the shift in fluorescence intensity of representative measurements are depicted. Error bars denote standard deviations. Significant differences (** p < 0.005, *** p < 0.001; Student’s t-test) are marked with an asterisk.
