Figure 6.

Binding of secreted REX variants in ELISA. The 96-well plate was coated with recombinant IL-23Rex protein, and samples of the medium with secreted REX proteins were applied (A). Purified protein REX115–TolA diluted in PBSTB was used as a positive control (B). The binding of REX proteins to IL-23Rex, and to BSA as a negative control, was detected by anti-DDDDK tag antibody [M2] conjugated with horseradish peroxidase (HRP) (A), or by streptavidin with HRP (B). Each bar represents the mean value with standard deviation and significant differences between mean values of the negative control (no REX), and the samples were analyzed by ANOVA (*** p < 0.004, **** p < 0.001, ns: no significant difference).