Table 1.
Fraction No. | Elute Solvent | Volume of Solvent | Weight of Fraction | Total Polyphenol Content | Total Anthocyanin Content | 50% Adsorption Inhibitory Concentration (IC50) | 50% Cytotoxic Concentration (CC50) | Selectivity Index |
---|---|---|---|---|---|---|---|---|
(mL) | (mg) | (µg/mL) | (µg/mL) | (µg/mL) | (µg/mL) | (CC50/IC50) | ||
Fruit (dry) | - | 5000 | - | - | - | - | - | |
Extract | - | 3077 | 423 | 297 | 72 | 159 | 2.2 | |
0 | Water | 200 | 2867 | 28 | 2 | ND | >400 | ND |
1 | 10% ethanol | 100 | 76 | 415 | 439 | ND | 236 | ND |
2 | 20% ethanol | 100 | 114 | 445 | 377 | 159 | >400 | >2.5 |
3 | 30% ethanol | 100 | 47 | 400 | 149 | 38 | 251 | 6.6 |
4 | 40% ethanol | 100 | 20 | 327 | 80 | 22 | 160 | 7.3 |
5 | 50% ethanol | 100 | 3 | 330 | 90 | 65 | 78 | 1.2 |
6 | 80% ethanol | 200 | 4 | 191 | 91 | 85 | 140 | 1.6 |
The initial material was extracted from 5 g of dried fruit using 80% ethanol, and its amount was calculated based on the amount of insoluble material. The extract and each fraction were adjusted to 1 mg/mL. ND—not detected.