Table 3.
Observed peaks in ESI–LC/MS analysis.
| Fraction | Peak | Retention Time | UV (λmax) | ESI Mode | Precursor Ion (Relative Intensity, %) |
Product Ion | Putative Compound |
|---|---|---|---|---|---|---|---|
| No. | (min) | (nm) | (m/z) | (m/z) | |||
| A | #1 | 7.10 | 279 | (−) | 451(32), 425(100), 407(41), 289(16) | 451, 425, 407, 289 | procyanidin B2 |
| (+) | 427(100), 409(63), 291(33) | 427, 429, 291 | |||||
| #2 | 7.45 | 329 | (−) | 295(8), 235(12), 217(55), 193(100), 175(47) | 295, 235, 217, 193, 175 | ferulic acid derivatives | |
| (+) | no information | no information | |||||
| #3 | 7.99 | 325 | (−) | 295(7), 235(12), 217(55), 193(100), 175(45) | 295, 235, 217, 193, 175 | ferulic acid derivatives | |
| (+) | no information | no information | |||||
| B | #4 | 11.05 | 315 | (−) | 193(100) | 193 | ferulic acid O-hexosides |
| (+) | 195(100), 163(45) | 195, 163 | |||||
| #5 | 11.46 | 307 | (−) | 193(100) | 193 | ferulic acid O-hexosides | |
| (+) | 195(100), 163(50) | 195, 163 | |||||
| C | #6 | 12.47 | 349 | (−) | 301(100) | 301 | quercetin O-rhamnoside |
| (+) | 303(100) | 303 | |||||
| #7 | 12.55 | 349 | (−) | 300(100) | 300 | Quercetin O-pentoside O-rhamnoside | |
| (+) | 449(100) | 449 |
Analysis was performed in the negative- and the positive-ion modes. Fraction A included procyanidin B2 and ferulic acid derivatives, and fractions B and C included ferulic acid hexosides and quercetin glycoside, respectively.