Figure 4.

SDS-PAGE and ELISA measurement of α- and γ-zein content in Oh51Ao2, Oh545o2, and their F₂ progeny with extremely high and low concentrations of eEF1A. A, For each lane, total zein extracts were prepared from 750 μg of endosperm flour of Oh51A+ and o2 and Oh545+ and o2 and separated by 12.5% (w/v) SDS-PAGE. M_r standards are shown on the right. To measure the amount of α- and γ-zein, samples were prepared from endosperm flour obtained from 20 kernels of the ear from an F₂ plant. Equal amounts of flour were mixed from the 10 high-eEF1A or the 10 low-eEF1A individuals to create the high- and low-eEF1A pools; triplicate measurements of zein proteins were made for each pooled sample. The α-zein (B) and γ-zein (C) contents were determined by ELISA as described in “Materials and Methods.”