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RNA-blot analysis showing α- and γ-zein transcripts in developing endosperm of Oh545o2 and Oh51Ao2. Total RNA was extracted from developing endosperm at 15 and 20 DAP, and 10 μg was separated by formaldehyde gel electrophoresis. After blotting on nylon membranes, the 19D α-, 22 α-, and 27 γ-zein transcripts were detected by hybridization with 32P-labeled cDNA probes. Sample loading was standardized with an 18S rDNA probe.
