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. 2019 Jun 11;19:250. doi: 10.1186/s12870-019-1867-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Identification of the lcd1 mutant by MutMap. a, Δ SNP-index plots of two pools on chromosome 7 generated by MutMap analysis. b, Structure of the OsNRAMP5 gene in lcd1 and WT. Gray boxes and black lines represent exons and introns in the OsNRAMP5 gene, respectively. SNP location is indicated below the gene structure with red font. c, Confirmation of SNP in the lcd1 mutant by Sanger sequencing. The SNP is indicated with red arrow. d, Homology comparison of the OsNRAMP5 protein in different crop plants. * indicates the identical amino acids, red box indicates the amino acid mutation location