Fig. 3.

DRBD2 is part of translational complexes in unstressed epimastigotes. Polysome fractionation by sucrose density gradient. The fractions (1–22) were collected after the sedimentation of cytoplasmic lysates from epimastigotes (a) in exponential growth and (b) subjected to nutritional stress, both treated with 100 mg/ml cycloheximide or 2 mM puromycin. The 40S and 60S ribosomal subunits, the 80S ribosome monomer, and polysomes are indicated. Western blotting of alternate fractions was performed with anti-protein A antibody (1:40,000 dilution) for TAP-tagged DRBD2 (52 kDa) detection, and mouse anti-S7 (25 kDa) antibody (1:1000 dilution) for S7 detection, a ribosomal protein commonly used as a control