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. 2019 Jun 6;14:4187–4209. doi: 10.2147/IJN.S198319

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© 2019 Gribko et al. This work is published and licensed by Dove Medical Press Limited

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Illustration of methods for CTC isolation by magnetic separation using magnetic nanoparticles (NPs).

Notes: (A) The CellSearch system enriches CTCs using iron NPs linked with anti-EpCAM antibodies (Abs). Labeled cells are magnetically separated, and can be evaluated by immunofluorescence staining. (B) AdnaTest allows the immunomagnetic enrichment of CTCs via epithelial and tumor-specific antigens. After separation of potential CTCs from PBMCs, labeled cells are lysed, in order to analyze CTC gene expression via multiplex PCR. (C) Process of MACS using superparamagnetic Fe NPs within a magnetized steel-wool column.

Abbreviations: CTCs, circulating tumor cells; PBMCs, peripheral blood mononuclear cells; MACS, magnetically activated cell sorting; RT, real time.