Table I.
Primer pairs used to amplify the extracellular domain of Lrk genes by PCR
| Specific Primer Sequence (5′ to 3′) | Ta | Lrk Gene | Chromosomal Location |
|---|---|---|---|
| G3 GAAAGATGAGTAAATTACTTG | 55°C | Lrk | 1A/1B/1D |
| UP1CTTGCAGTCCAACGGAAG | |||
| 14F1 ATGTCGCGAATCATGGAC | 51°C | CSLrk17 | 1D |
| 14R3 GTTTGTAGTCTTCGTACA | |||
| G3/19R1 GTCCATTCGAAAAATTG | 49°C | CSLrk6 | 1B |
| G3/25R1 GAATTCCATAGGTAAAG | 49°C | CSLrk9 | 1B |
| G3/CS3 TGAGAATTGCCGATCACG | 53°C | CSLrk3 | 1D |
| G3/CS16 GGGCCAGCAATGCTTTC | 55°C | CSLrk16 | 1B |
| G3/CS11 GGGTTGCAGATGAACCCTC | 55°C | CSLrk11 | 1B |
| G3/CS13 CAGAACTCCAAAGAAATATC | 50°C | CSLrk13 | 1A |
| G3/CS5 GGTTGAAGATGAACTATC | 48°C | CSLrk5 | 1A |
| G3/CS41 GAGTGCACTAGGGTTGCAGATGA | 50°C | CSLrk41 | ? |
| UP1/CS22 CCTCCTGCTGCTACCTCTTAC | 55°C | CSLrk22 | 1D |
G3 and UP1 are located in very conserved regions of the Lrk genes and allow the amplification of all Lrk genes on chromosome group 1 in wheat. They can be used in combination with specific primers to amplify specifically one member of the gene family (CSLrk) and to determine the localization on the homoeologous chromosomes 1A, 1B, and 1D with aneuploid wheat lines. The annealing temperature (Ta) is indicated.