FIG 3.

Antofine targets RRD2 and disrupts the Tap42-phosphatase complex in F. graminearum. (A) Model representing the TOR complex with components of the Tap42-phosphatase complex highlighting the negative regulation of RRD2 by antofine. The phosphatases are colored green. (B) Antofine specifically disrupts FgTap42-FgRRD2 protein interaction in the Tap42 complex. The gene pairs FgTap42 and FgRRD2 and FgTap42 andFgPpg1 were expressed in yeast in the absence (−) and in the presence of antofine and tylophorine at 0.1 and 1 μg/ml. The β-galactosidase specific activity (in Miller units) was measured by the ONPG assay (23). (C) The interaction between Tap42 and FgPpg1 has been demonstrated previously and was used as a positive control (11). Shown is a representative of three independent biological replicates, each with three technical replicates.