FIG 2.

Characterization of camphor-regulated ERG11 allele. (A) A diagram showing the control of the camR-regulated camPr-ERG11 fusion gene. In the absence of camphor, camR-VP16 can bind to and induce camPr-ERG11 expression. The presence of camphor causes camR-VP16 to be released from this promoter, and ERG11 expression is strongly inhibited. (B) The presence of the camPr-ERG11 allele confers a camphor-sensitive growth phenotype. Strains containing both ScTDH1-driven camR-VP16 and camPr-ERG11 (BVGC9) or only ScTDH1-driven camR-VP16 (BVGC7) were grown overnight and then diluted into media containing or lacking camphor as indicated. Cultures were allowed to grow at 30μC, and optical density was measured using a spectrophotometer. (C) Mid-log phase cultures of BVGC9 grown in the absence (Untreated) or the presence (Treated) of 50 μM camphor for 2 h were harvested and ergosterol levels measured as described previously (41). (D) Cultures of the indicated strains were grown to the mid-log phase and then diluted for plating on rich media containing the listed additions. “Vehicle” represents the solution used to solubilize ergosterol.