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. 2019 Jun 11;10(3):e00934-19. doi: 10.1128/mBio.00934-19

FIG 5.

FIG 5

Camphor-off alleles of other ERG genes also lead to induction of PDR1 and CDR1. (A) Three additional strains were produced that contained both the camR-VP16 transactivator and the camphor-off alleles of ERG1, ERG2, or ERG3. These strains, along with a wild-type control, BVGC9 (camR-VP16, camPr-ERG11) were grown to the mid-log phase and serial dilutions plated on rich medium (YPD) or YPD containing 50 μM camphor. Plates were then incubated at 30μC and photographed. (B) The camphor-repressible ERG2 fusion gene-containing strain was grown and left untreated or challenged with camphor for the indicated times. Total RNA was prepared from each sample and analyzed for expression of ERG2, PDR1, and CDR1 mRNA by qRT-PCR. (C) The transcriptional response of the ERG pathway genes was determined by qRT-PCR from cells grown as described for panel B. (D) The strain with the camphor-repressible ERG3 gene was grown in the absence or presence of camphor as described above followed by the indicated qRT-PCR analyses. (E) The transcriptional responses of the ERG pathway to camphor-mediated ERG3 repression determined across the indicated ERG pathway genes.