Figure 7: Efficiency and selectivity of AAV1.S2a gene delivery to the lung.
A. Transgene copies were assessed by RT-PCR in RV and lung tissue samples to determine the efficiency and specificity of AAV1.S2a gene transfer to the lung. N=5-6 rats per group. ** P<0.01. B. Human and rat SERCA2a expression measured by RT-PCR in RV samples from CTRL, MCT, and MCT+S2a hearts. C. Representative immunofluorescence images and expression of SERCA2a (red) relative to α-actinin (green) in RV tissue sections from CTRL, MCT, and MCT+S2a hearts. D. Representative western blots of SERCA2a and GAPDH as a loading control, and quantification of relative SERCA2a protein expression in the RV of CTRL, MCT, and MCT+S2a hearts.