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. 2019 Jun 2;25:4094–4103. doi: 10.12659/MSM.914202

Figure 3.

Figure 3

miR-466 directly targeted NUS1. (A, B) Predicted miR-466 target sequences in the 3′UTR of NUS1 and the mutant containing altered nucleotides in the 3′UTR of NUS1. (C) Confirmation of the effectiveness of pri-miR-466 and ASO-miR-466 by RT-qPCR in H8 and End1/E6E7 cells. (D) EGFP assay of the indicated cells transfected with pcDNA3/EGFP-NUS13′UTR reporters and the indicated plasmids. (E) EGFP assay of H8 and End1/E6E7 cells transfected with pcDNA3/EGFP-NUS1 3′UTR mut reporters and the indicated plasmids. (F) Confirmation of the NUS1 mRNA level transfected with the indicated plasmids by RT-qPCR assay. (G) Confirmation of the relative protein expression levels of NUS1transfected with the indicated plasmids by Western blot assay. Results are presented as the mean ±S.D from 3 independent experiments. * P<0.05, ** P<0.01, and *** P<0.001.