Table 1.
RT-qPCR primers | Primer sequence (5′-3′) |
---|---|
miR-466-RT | GTCGTATCCAGTGCAGGGTCCGAGGT |
GCACTGGATACGACATGTGTGT | |
miR-466-qPCR-Fwd | TGCGGCATACACATACACGC |
Oligo-dT | TTTTTTTTTTTTTTTTTT |
U6-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTC |
GCACTGGATACGACAAAATATGGAAC | |
U6-Fwd | TGCGGGTGCTCGCTTCGGCAGC |
miRNA-universal-Rev | CCAGTGCAGGGTCCGAGGT |
NUS1-qPCR-Fwd | ACAGCGTCTGGGAAAGTA |
NUS1-qPCR-Rev | AGTAGCTGGGCTGAAGAC |
β-actin-qPCR-Fwd | CGTGACATTAAGGAGAAGCTG |
β-actin-qPCR-Rev | CTAGAAGCATTTGCGGTGGAC |