Table 3.
Spontaneous bz mutations not caused by a retrotransposon insertion
| bz mutant allele | Nature of mutation | Relevant details | Bz site location (from ATG) |
| bz-B73:N12 | 264-bp MULE | 9-bp TSD | 279–287 exon 1 |
| bz-B73:N14 | 156-bp deletion | 7-bp microhomology at junction | 627–782 exon 2 |
| bz-B73:N27 | 8-bp insertion | TCA insertion between 5-bp TSD | 660–661 exon 2 |
| bz-B73:N28* | 19-bp deletion | 6-bp microhomology at junction | 339–357 exon 1 |
| bz-B73:N29* | 19-bp deletion | 6-bp microhomology at junction | 339–357 exon 1 |
| bz-McC:N3 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-McC:N4 | 1,028-bp deletion | 5 bp at junction = Hopscotch end | 1434–2461 exon 2 + 3′ end |
| bz-McC:N5 | 905-bp deletion | 7/8-bp homology at junction | −321 to 585 exon 1 |
| bz-McC:N6 | 320-bp duplication | Complex: three new junctions | 1151–1248, 929–1150 exon 2 |
| bz-McC:N7 | 777-bp deletion | 4-bp microhomology at junction | 632–1408 exon 2 |
| bz-McC:N9 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-McC:N10 | 860-bp deletion | 2-bp microhomology at junction | 317 exon 1 to 1176 exon 2 |
| bz-McC:N11 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-McC:N12 | 467-bp deletion | No microhomology at junction | 99 exon 1 to 565 intron |
| bz-McC:N13 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-McC:N14 | 1,679-bp deletion | No microhomology at junction | −1641 (stk1) to 38 bz exon 1 |
| bz-McC:N17† | 1-bp change | G392 to D392 | 1275 in exon 2 |
| bz-McC:N23† | 1-bp change | G392 to D392 | 1275 in exon 2 |
| bz-McC:N24‡ | 8-bp insertion | TAA insertion between 5-bp TSD | 149–150 exon 1 |
| bz-McC:N25† | 1-bp change | G392 to D392 | 1275 in exon 2 |
| bz-McC:N26‡ | 8-bp insertion | TAA insertion between 5-bp TSD | 149–150 exon 1 |
| bz-McC:N27 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-McC:N28 | 104-bp deletion | No microhomology at junction | 335–439 exon 1 |
| bz-McC:N30 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-McC:N31 | 582-bp deletion | 19-bp unknown filler in junction | 688–1269 exon 2 |
| bz-McC:N32 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-McC:N35 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
| bz-4Co63:N6 | 1-bp change | C942 to A942 creates stop | 942 in exon 2 |
| bz-4Co63:N9 | >3-kb deletion | No PCR product with bz primers | Entire bz gene |
*
These two mutant alleles had identical mutations.
†
These three mutant alleles had identical mutations.
‡
These two mutant alleles had identical mutations.