Fig. 2.

Cnd3 K870 is the responsible SUMOylation target. (A) Potential SUMOylation target sites in Cnd3 identified in a proteome-wide study (43) or predicted by GPS-SUMO software (86). Cnd3 K870 was identified by both methods. (B) K870 may be the sole SUMOylation target in Cnd3, as the Cnd3 SUMOylation band (upper band) disappeared in the cnd3-K870R mutant. The cs mutant, nda3-KM311, was used to arrest cells in mitosis (20 °C, 8 h). (C) cnd3-K870R rescues the cnd2-A708V ts mutant, which resembles those of ∆pli1 in Fig. 1E. (D) Conservation of Cnd3 K870 among four fission yeast species: S. pombe, Schizosaccharomyces octosporus, Schizosaccharomyces japonicus, and Schizosaccharomyces cryophilus.