Fig. 4.

AX promotes neuronal cell viability with LEP induction. (A) AX treatments of 10 and 20 μM in Opti-MEM medium alone without H₂O₂ effectively and significantly decrease LDH release compared with the DMSO group (n = 3/group). (B) Western blots showing that 10 and 20 μM of AX significantly increases LEP expression compared with DMSO (n = 4–5/group). Protein levels normalized to the band intensity of β-actin. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01, vs. DMSO. NS, not significant.