Figure 3.

BACRF analysis of pSB1742-hybridizing BACs. The sorghum genomic clone pSB1742 detects RFLPs that map to LGs B (allele pair indicated by a, one of which comigrates with BAC vector), C (single band designated b in SP), and G (single band designated c in SB), as well as several bands that could not be mapped (such as d). Lanes 1 through 13 are HindIII digests of pooled BAC DNA extracted from 384 clones each, according to Lin et al. (2000). The pSB1742b locus on LG C corresponds to a BAC in pool 13, proven by dot blots (not shown) to be BAC 13I24. A second BAC in pool 12 (12A22) corresponded to an unmapped locus. Other corresponding BACs are not shown.