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Low relative humidity predisposes Mx1 congenic mice to influenza disease. Mx1 congenic mice were preconditioned at 20% and 50% RH for 5 d and then challenged with aerosolized hvPR8 at 2 × 105 pfu/mL. (A) Weight loss, (B) core body temperature, and (C) survival were monitored for 11 d (n = 10 mice per group, pooled from two independent experiments). Data are representative of five experiments and means ± SEM *P < 0.05; one-way ANOVA; log-rank (Mantel–Cox).
