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Fine neuronal structure preserving by FOCM. (A) Three-dimensional reconstruction of neuron dendrites before FOCM clearing (Upper) and after clearing (Lower). (B) Images of dendrites (Left) and synaptic buttons (Right) at the depth of 0, 20, and 40 μm before clearing. (C) Images of dendrites (Left) and synaptic buttons (Right) at the depth of 0, 20, and 40 μm after FOCM clearing. (Scale bars: C, Left, 10 μm; C, Right, 1 μm.)
