Fig. 7.

FOCM realized a fast-imaging spatial merging. (A) Experimental design for CT-B injection at NAc in C57BL/6 mouse (9 wk old) slices. (B) Maximum intensity projection (MIP) in insular cortex. (C and D) Selected single imaging at depth of 12 µm (C) and 56 µm (D) in white box in B. (E) Three-dimensional reconstruction of the white region in B. Blue dash boxes correspond to C and E. (F) Specific position of C and D in reconstruction results. (G) Specific position of cell body model corresponding to the cell in yellow boxes in C and D. Red represents c-fos–positive cell and green represents CT-B–positive cell. (Scale bars: 20 µm.)