[18F]DASA-23 accumulation in HeLa cells transfected with a plasmid containing the PKM2 gene, GFP gene, and nontransfected control cells. Radiotracer accumulation was measured after 30 and 60 min of tracer incubation, as well as 60 min incubation followed by 30 min efflux (n = 3, error bars represent SD); 30 min PKM2 vs. GFP (P < 0.001, ***), PKM2 vs. control (P < 0.0001, ****), GFP vs. control (P > 0.05, ns). 60 min PKM2 vs. GFP (P < 0.01, **), PKM2 vs. control (P < 0.01, **), GFP vs. control (P > 0.05, ns). Efflux PKM2 vs. GFP (P > 0.05, ns), PKM2 vs. control (P < 0.05, *), GFP vs. control (P > 0.05, ns).