Fig. 2.

dATP promotes motility of unregulated F-actin across a range of ionic strengths. (A) Fraction of sliding actin filaments is significantly greater across a range of ionic strengths for dATP-bound myosin (blue bars) compared with ATP-bound myosin (white bars). (B) (Nonerratic) filament sliding velocity (Vel.) is also significantly increased across a wide range of ionic strengths for dATP compared with ATP, likely owing to a combination of increased ATPase rates and increased actin affinity of dATP-bound myosin compared with ATP. *P < 0.05 with an unpaired Student’s t test. Error bars represent SEM for n ≥ 12 slides.