Fig. 2.

The expression of OXPHOS complexes controls CL remodeling in yeast. (A) Yeast strains were grown in YPD or YPGE and harvested in the midlogarithmic (log) or in the stationary (stat) phase. The average number of double bonds per CL molecule and the MLCL/CL ratio were determined by MS. Data are mean values ± SEM (n = 3). (B) Yeast cells and their corresponding rho⁰ strains were grown in YPD and harvested in stationary phase. Lipids were analyzed by MS to determine the proportion of diunsaturated (CL∆2), triunsaturated (CL∆3), and tetraunsaturated (CL∆4) CL species as well as the MLCL/CL ratio. Data are mean values ± SEM (n = 4). (C) Yeast strains with the indicated deletions (SI Appendix, Table S1) were grown in YPD to stationary phase, and CL was analyzed by MS. Data are mean values ± SEM (n = 3). Asterisks indicate a statistically significant difference to the wild type (P < 0.05, χ² test).