Table 1.
Biophysical properties of Adk mutants
| Mutant | ΔTm, °C* | kcat, μM ADP/min/nM Adk† | KM, μM AMP† | KI, μM AMP†,‡ | Distance from AP5A, Å |
| V106H | −11.7 | 93.2 (2.1) | 133.6 (9.5) | NA§ | 13.4 |
| V106W | −9.9 | 72.6 (4.6) | 195.7 (35.3) | NA§ | 13.4 |
| L82F | −5.7 | 87.3 (6.9) | 84.8 (13.7) | 2,302.0 (738.2) | 10.5 |
| A93F | −5.5 | 87.0 (8.6) | 91.3 (16.5) | 889.1 (209.7) | 8.7 |
| V106A | −5.2 | 72.0 (6.5) | 58.0 (11.1) | 1,287.0 (355.5) | 13.4 |
| V106L | −4.8 | 106.6 (2.4) | 190.5 (12.2) | NA§ | 13.4 |
| A93L | −4.3 | 80.8 (3.4) | 119.8 (16.5) | NA§ | 8.7 |
| A93Y | −3.7 | 85.7 (7.9) | 97.0 (18.0) | 3,046.0 (1,349.0) | 8.7 |
| A93I | −3.6 | 69.0 (5.6) | 349.8 (67.8) | NA§ | 8.7 |
| L83I | −2.5 | 105.9 (5.4) | 259.1 (34.7) | NA§ | 8.8 |
| WT | 0.0 | 68.6 (3.6) | 46.8 (5.2) | 930.5 (154.7) | |
| Y182F | 0.5 | 88.1 (7.0) | 87.0 (14.0) | 2,365.0 (766.8) | 10.7 |
| L83F | 0.6 | 73.9 (4.5) | 94.1 (9.6) | 680.2 (106.1) | 8.8 |
| E210L | 0.7 | 85.8 (7.3) | 115.1 (15.7) | 638.1 (132.1) | 13.1 |
| L82V | 0.8 | 28.5 (8.3) | 36.2 (17.0) | 76.6 (33.4) | 10.5 |
| V169E | 1.8 | 63.1 (4.0) | 83.6 (9.2) | 595.9 (92.9) | 10.0 |
| L209I | 2.6 | 36.9 (2.0) | 32.7 (3.7) | 382.9 (45.0) | 9.4 |
| M21A | 2.8 | 68.5 (6.4) | 48.2 (9.4) | 729.5 (166.3) | 12.5 |
| V169E + L209I | 2.8 | 71.8 (3.6) | 38.5 (4.0) | 460.5 (54.2) | |
| L107I | 3.2 | 23.6 (1.4) | 22.4 (3.2) | 511.7 (77.4) | 9.5 |
| M21A + V169E | 3.5 | 60.4 (2.5) | 43.3 (3.6) | 456.4 (44.3) | |
| L107I + V169E | 3.5 | 97.6 (5.6) | 60.6 (6.3) | 453.0 (57.6) | |
| M21A + L209I | 3.8 | 55.7 (2.7) | 25.5 (2.6) | 227.4 (20.7) | |
| M21A + L107I | 4.3 | 72.2 (5.6) | 43.2 (6.0) | 211.3 (28.1) | |
| M21A + V169E + L209I | 4.7 | 64.0 (2.5) | 31.6 (2.5) | 228.9 (16.4) | |
| M21A + L107I + V169E | 5.2 | 68.8 (3.7) | 37.7 (4.0) | 308.6 (32.9) | |
| Q16Y | 5.4 | 4.9 (1.1) | 186.3 (118.3) | NA§ | 5.1 |
| Q16F | 6.5 | 10.6 (1.4) | 324.2 (101.0) | NA§ | 5.1 |
| Q16F + V169E | 7.3 | 20.6 (3.9) | 479.3 (156.5) | NA§ | |
| Q16F + L107I | 8.1 | 21.9 (2.7) | 286.8 (74.2) | NA§ | |
| Q16F + L107I + V169E | 8.5 | 14.0 (2.8) | 241.0 (107.0) | NA§ | |
| Q16F + M21A | 8.7 | 24.0 (3.4) | 491.7 (118.4) | NA§ |