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. 2019 May 16;116(23):11418–11427. doi: 10.1073/pnas.1815166116

Fig. 2.

Fig. 2.

The up-regulation of Galnt3 by Pi requires activation of the ERK pathway and induction of Egr1 and Etv5. (A and B) The results of DNA microarray analysis in a volcano plot using UMR106 cells with or without 5 mM Pi treatment for 6 h (A, Left). The table shows 17 genes significantly induced by Pi (A, Right). These included genes for several transcription factors, ERK pathway components, and bone-related proteins (B). (C) Egr1, Etv4, and Etv5 mRNA expression by 5 mM Pi or 5 mM sulfate for 6 h evaluated by qPCR. (D) Galnt3 mRNA expression by 5 mM Pi for 12 h compared with 1 mM Pi under siRNA-mediated silencing of Egr1, Etv4, and Etv5. (E and F) Immunoblotting with an antibody to phosphorylated ERK1/2 from the lysates of UMR106 cells treated with 1 or 5 mM Pi, 100 ng/mL FGF2, or 5 mM sulfate for 15 min (E) or treated with 1, 3, or 5 mM Pi (F). (G) ERK activation under various extracellular Pi concentrations for 24 h evaluated by SRE luciferase assays. (H) Galnt3 mRNA expression by 5 mM Pi for 48 h with or without the MEK1/2 inhibitor U0126. Data represent the mean ± SEM. (C, D, and G) n = 3 per group; *P < 0.05 by ANOVA with a post hoc Tukey’s test (C and D) or with a post hoc Dunnett’s test compared with 1 mM of extracellular Pi (G). (E and F) Data are presented as a representative image. (H) n = 3 per group; *P < 0.05 compared with 1 mM Pi without U0126, #P < 0.05 compared with 1 mM Pi with U0126, †P < 0.05 compared with 5 mM Pi with U0126 cells by ANOVA with a post hoc Tukey’s test.