Table 1.

Five most significantly affected canonical pathways by miR-328 knockdown predicted using IPA (Ingenuity Pathway Analysis software, Ingenuity Systems, www.ingenuity.com). A) Pooled proteomics setup. B) Separated proteomics setup.

Proteomics setup	Top canonical pathways	p-value
A) Pooled
Soluble fraction	eIF2 Signaling	1.33E−48
	Regulation of eIF4 and p70S6K Signaling	1.16E−32
	Mitochondrial Dysfunction	3.19E−28
	Protein Ubiquitination Pathway	2.46E−27
	mTOR Signaling	2.31E−23
Microsomal fraction	eIF2 Signaling	1.33E−48
	Regulation of eIF4 and p70S6K Signaling	1.16E−32
	Mitochondrial Dysfunction	3.19E−28
	Protein Ubiquitination Pathway	2.46E−27
	mTOR Signaling	2.31E−23
B) Separated
Soluble fraction	eIF2 Signaling	9.79E−56
	Regulation of eIF4 and p70S6K Signaling	1.90E−35
	Protein Ubiquitination Pathway	5.23E−26
	Mitochondrial Dysfunction	1.49E−23
	mTOR Signaling	1.40E−20
Microsomal fraction	eIF2 Signaling	9.79E−56
	Regulation of eIF4 and p70S6K Signaling	2.12E−36
	Mitochondrial Dysfunction	1.84E−35
	Protein Ubiquitination Pathway	1.72E−30
	mTOR Signaling	4.53E−26