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. 2019 Jun 5;13:184. doi: 10.3389/fncel.2019.00184

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Copyright © 2019 Harris, Masgutova, Collin, Toch, Hidalgo-Figueroa, Jacob, Corcoran, Francius and Clotman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Onecut factors are present in multiple subsets of V2 interneurons. (A–I”) Immunolabelings for OC, the V2a generic marker Chx10 and markers of V2a subpopulations (Francius et al., 2013) on transverse spinal cord sections (brachial or thoracic levels) of e12.5 wild-type mouse embryos. In each figure, the right ventral quadrant of the spinal cord is shown. Only HNF-6 is detected in Shox2+ V2a cells (arrow in A–C”), whereas the three OC are present in the MafA+ and in the cMaf+ V2a subsets (arrows in D–I”). (J–L”) Immunolabelings for OC, the V2b generic marker Gata3 and MafA. The three OC proteins are detected in MafA+ V2b interneurons (arrows). (M–O”) Immunolabelings for OC and the V2c marker Sox1 demonstrate that OC factors are present in a majority of V2c interneurons (arrows). Sox1 in the ventricular zone labels neural progenitors. Scale bar = 50 μm.