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. 2019 Jun 5;13:184. doi: 10.3389/fncel.2019.00184

FIGURE 6.

FIGURE 6

The Pou2f2+ V2a interneurons are mislocated in the absence of OC factors. (A–F) Immunolabelings and quantification of Pou2f2+ V2a interneurons in control or Hnf6-/-;Oc2-/- mutant embryos. At e12.5 (A,B) and e14.5 (C,D), Pou2f2 is detected in V2a Chx10+ interneurons, and the number of Pou2f2-containing Chx10+ cells trends to increase but is not significantly different in the absence of OC factors (E,F). (G–DD) Distribution of Pou2f2+ V2a interneurons on the transverse plane of the spinal cord in control or Hnf6-/-;Oc2-/- double-mutant embryos. One-dimension graphs (lower) show density distribution on the dorso-ventral (left) or the medio-lateral (right) axis of the spinal cord. (G–R) At e12.5, cells in the central clusters are slightly reduced at brachial and at thoracic levels in the absence of OC factors (n = 3, p ≤ 0.001 at brachial and thoracic levels; p = 0.41 at lumbar level). (S–DD) At e14.5, a vast majority of V2a containing Pou2f2 settles in a more medial position in Hnf6-/-;Oc2-/- spinal cords (n = 3, p ≤ 0.001). Mean values ± SEM. Scale bar = 50 μm.