Skip to main content
. 2019 Jun 5;13:184. doi: 10.3389/fncel.2019.00184

FIGURE 7.

FIGURE 7

The Pou2f2+ V2b interneurons are mislocated in the absence of OC factors. (A–F) Immunolabelings and quantification of Pou2f2+ V2b interneurons in control or Hnf6-/-;Oc2-/- mutant embryos. At e12.5 (A,B) and e14.5 (C,D), Pou2f2 is present in V2b Gata3+ interneurons, but the number of Pou2f2+ V2b cells was not significantly increased in the absence of OC factors (E,F). (G–DD) Distribution of Pou2f2+ V2b interneurons on the transverse plane of the spinal cord in control or Hnf6-/-;Oc2-/- double-mutant embryos. One-dimension graphs (lower) show density distribution on the dorso-ventral (left) or the medio-lateral (right) axis of the spinal cord. (G–R) At e12.5, Pou2f2-containing V2b are more central and slightly more ventral in the absence of OC factors (n = 3, p ≤ 0.001). (S–DD) At e14.5, this subset of V2b interneurons is more clustered on the medio-lateral axis in Hnf6-/-;Oc2-/- spinal cords (n = 3, p ≤ 0.001). Mean values ± SEM. Scale bar = 50 μm.