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. 2019 Jun 5;13:184. doi: 10.3389/fncel.2019.00184

FIGURE 8.

FIGURE 8

V2 interneuron distribution is altered after misexpression of Pou2f2. Overexpression of Pou2f2 in chick embryonic spinal cord after electroporation at HH14-16 and immunolabelings 72 h after electroporation. (A–F) At HH27-28, Pou2f2 overexpression does not impact the number of V2a (E) or V2b (F) interneurons. In contrast, it alters V2 distribution. (G–J) In control spinal cord, V2a interneurons are distributed in two closely connected clusters on the medio-lateral axis of the neuroepithelium. In electroporated spinal cord, lateral migration is increased and a majority of V2a interneurons are clustered in a single central group with ectopic lateral extensions (arrows; n = 3, p ≤ 0.001). (K–N) In control spinal cord, V2b are distributed in two groups along the medio-lateral axis with a majority of cells in the lateral cluster. In electroporated spinal cord, the V2b interneurons are equally distributed between these two clusters (n = 3, p ≤ 0.001). Mean values ± SEM. Scale bar = 50 μm.