Table 2:
Conversion of 8PN by E. ramulus and IX by E. ramulus and E. limosum co-cultures.
Starting metabolite | |||
IX | 8PN | DDXN | |
E. ramulus | |||
8PN | ND* | 15.5% (1.64%) | 55.7% (24.59%) |
E. ramulus + E. limosum | |||
IX | 7.6% (2.17%) | 34.5% (1.99%) | 63.3% (8.08%) |
8PN (50 μM) was added to a culture of E. ramulus and IX (50 μM), to a co-culture of E. ramulus and E. limosum. After an incubation period of 3 days, the concentrations of IX, 8PN and DDXN were measured. On day 0, the average recovery of 8PN and IX was 17 μM and 20 μM, respectively. The results are presented as mean (+SEM) percentage recovery of IX, 8PN and DDXN calculated as molar percentages of the day 0 concentrations of the flavonoids.
ND: not detectable.