Figure 1.

Transposon-mediated generation of single-copy transgenic plants. DNA-blot hybridization shows the integration pattern of Ds-bar after HindIII digest of genomic DNA from F₁ parent, A18-5 (far left lane), and 20 of its F₂ progeny. In three plants (i.e. nos. 3, 6, and 8), a single Ds-bar cassette has transposed to an unlinked site, segregating away from other copies. The asterisk above the band marks the position in single-copy Ds-bar plants that differs from the parental bands. M_r markers in kilobases are indicated on the left.