Figure 2.

Average frequency of transgene expression instability in F₂, F₃, and F₄ populations. Lines from F₂, F₃, and F₄ populations were assessed for functional expression of the product of bar by testing for Basta sensitivity. Percentages represent the number of lines in which at least one plant exhibited Basta sensitivity divided by the total number of lines per family tested. TNP, Transposed Ds-bar element, no ActPase; nTNP, Ds-bar element remaining at original site of integration following bombardment, no AcTPase; TGE, transgene expression.