Figure 3.

Analysis of Ds-bar integration sites. Genomic DNA flanking single-copy Ds-bar elements was isolated from six different transgenic plants by thermal asymmetric interlaced-PCR. PCR fragments were then used as hybridization probes with DNA blots of HindIII-digested genomic DNA from non-transgenic plants. The two lanes on left are results after hybridization with a probe specific to the genomic DNA flanking the original Ds-bar insertion site of line A8-1-5. Other sets of lanes show results after hybridization with probes flanking the insertion sites from five lines in which Ds-bar has transposed to a new location: four lines that stably express the transgene, e.g. nos. 22, 34, 85, 120, and one line that does not, 51. M_r markers in kilobases are indicated on left.