Table I.
Transgene expression stability in nTNP and TNP plant lines
| F1 Plant | No. of Copies | No. of Lines with Transgene Expression Instability/Total Lines Analyzed
|
|||||
|---|---|---|---|---|---|---|---|
| F2
|
F3
|
F4
|
|||||
| nTNPa | TNPb | nTNP | TNP | nTNP | TNP | ||
| A8-1 | 1 | 6/8 (75%) | 0/15 (0%) | 6/8 (75%) | 2/15 (13%) | 7/8 (88%) | 2/15 (13%) |
| A8-5 | 1 | 4/5 (80%) | 0/10 (0%) | 4/5 (90%) | 2/10 (20%) | 5/5 (100%) | 2/10 (20%) |
| A1-5 | 6 | N.D. | N.D. | 2/2 (100%) | 1/5 (20%) | 2/2 (100%) | 2/5 (40%) |
| A18-5 | 5 | N.D. | N.D. | 2/2 (100%) | 1/5 (20%) | 2/2 (100%) | 1/5 (20%) |
a
nTNP lines are “non-transposed,” containing Ds-bar at the original integration site and do not contain AcTPase.
b
TNP lines are “transposed” and are progeny of F1 plants selected for a single copy of Ds-bar transposed to a new location.