Figure 4. Flies Fed a High Sugar Diet Show Increased Feeding Behavior, Meal Size, and Duration.

(A) Average licks per day of age-matched w¹¹¹⁸CS male flies feeding continuously on 5% (salmon) or 20% (burgundy) sucrose on the FLIC. n = 26–72, two-way ANOVA with Fisher’s LSD, compared to same-day 5% sucrose licks.

(B) Heatmap of the licks binned by 30 minutes of individual flies (left y axis) feeding continuously on 5% or 20% sucrose on the FLIC at days 1, 2, 5, and 7. The x axis represents time in 24 h, time 0 indicates midnight (Zeitgeber time 17 [ZT17]).

(C) Meal patterns quantified as average licks binned by 30 min for flies feeding on 5% or 20% sucrose on selected days 1, 2, 5, or 7 (from A). The x axis is as in (B).

(D) Schematic for how meal duration and size were determined for morning (a.m.) and evening (p.m.) meals.

(E and F) The meal (E) duration (h) and (F) size in licks of the morning (a.m.) and evening (p.m.) meals of flies feeding on 5% or 20% sucrose on day 1 (solid bars), day 5 (spotted bars), and day 7 (hatched bars). n = 23–65, two-way ANOVA with Fisher’s LSD, comparisons to same-diet day-1 meal-time duration or size.

All data shown as mean ± SEM, ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05 for all panels unless indicated.

See also Figure S4.